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Chroma Technology (filter/spectra information)
System Reccomendations
Microscope |
B29 Confocal LSM 510 |
Confocor III |
Marianas |
Axiovert |
Axioplan |
Laser Lines |
405,488,543,633 |
488,543,633 |
Tirf laser for RFP, M-Cherry, & GFP Arc lamp for other widefield |
Arc lamp |
Arc Lamp |
Recommended Fluorophores
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DAPI/HOECHST FITC, Alexa 488, GFP TxRed, Cy3, Alexa 546 Alexa 633-647/Cy5 |
FITC, Alexa 488, GFP TxRed, Cy3, Alexa 546 Alexa 633-647/Cy5
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Simultaneous Capture on RFP/GFP & CFP/YFP DAPI FITC Cy3 Cy5 |
DAPI FITC TxRed |
DAPI CFP/YFP/FITC TRITC/RFP/TRITC Cy5 Fura2 |